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RTD6137非变性电泳蛋白质Marker(45-880 kD)
 产品货号: RTD6137
 产品名称: RTD6137非变性电泳蛋白质Marker(45-880 kD)
 产品价格/规格: 20次 100μl 500元
 产品说明书: 点击查看
 更新时间: 2022-07-29
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  • 非变性电泳蛋白质Marker45-880 kD

    Native Electrophoresis Protein Marker45-880 kD

    ●产品编号及规格:

    RTD6137   20次 100 μl

    ●储存及运输条件:

    -20℃保存,湿冰运输,有效期为12个月。

    产品简介:

        本产品有4种蛋白组成,分子量范围为45-880 kD,经过非变性电泳后,用考马斯亮蓝染色后可以得到5条主带。

    蛋白名称

    分子量(kD)

    蛋白来源

    pI

    说明

    Ferritin

    440

    880

    equine spleen

    N/A

    非变性下440kD880 kD

    Carbonic Anhydrase

    300

    Bovine Erythrocytes

    6.4

    单体分子量29kD,非变性下为10聚体,低于300kD有电荷异构体(charge isomer

    Albumin

    66

    bovine serum

    4.6-5.8

    球蛋白,单体分子量66 kD,非变性下可形成少量二聚体

    Ovalbumin

    45

    egg white

    4.71

    4.59

    球蛋白,分子量为45kD,非变性条件下大于45kD会出现电荷异构体(charge isomer

    使用说明:

    1. 取出产品后,常温融化后,彻底混匀,上样电泳。

    注:上样量根据胶的厚度和梳子的宽度确定。一般说来,1.0厚度10齿梳子加样孔上样5 μl1.0厚度15齿梳子加样孔上样量2.5 μl,其他规格梳子请适当调整上样量。

    2. 该产品不含蛋白凝胶制备试剂,您可以选择非变性PAGE凝胶制备及电泳试剂盒(货号:RTD6130)或者蛋白预制胶(货号RTD6118)。如自备试剂,经典Laemmli系统中把制胶溶液、电泳缓冲液和上样缓冲液中的SDS和还原剂(DTT或β-巯基乙醇)全部去除即可进行非变性电泳。

    3. 电泳条件:

    建议使用8%非变性胶(货号:RTD6118-0008)或者4-12%梯度非变性胶(货号:RTD6118-0412)进行电泳。

    恒电压

    120 V

    起始电流

    27-35 mA/板胶

    结束电流

    7-10 mA/板胶

    电泳时间

    1h20min+

    4. 电泳结束后,考马斯亮蓝染色,观察结果。

    注:使用银染染色时,由于灵敏度高于考马斯亮蓝染色方法,需要适当降低Marker上样量,一般稀释50倍后上样。

    注意:

    1. 本蛋白Marker不适用于变性蛋白电泳(SDS-PAGE),因为在SDS存在下,含有多个亚单位的蛋白会不同程度解聚。

    2. 在非变性条件下,蛋白的迁移与蛋白的电荷、蛋白形状以及蛋白分子量都有关。因此,在一种凝胶浓度下使用本Marker不能精确估计出目的蛋白的分子量。非变性电泳中,蛋白分子量的确定应该是在不同凝胶浓度下,确定出蛋白的Rf值,绘制出凝胶浓度对Rf的曲线从而判定蛋白的分子量。

    ● 实验示例:

     




  • 使用蛋白Marker产品发表部分文章列表:

    1. [2021 IF=5.5] Garviecin LG34, a novel bacteriocin produced by Lactococcus garvieaeisolated from traditional Chinese fermented cucumber.

    Author: Yurong Gao, Dapeng Li, Shan Liu, Liyuan Zhang

    Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

    Journal: Food Control 50 (2015) 896e900

    Institution:Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

    Paper linkhttp://dx.doi.org/10.1016/j.foodcont.2014.10.040

     

    2. [2021 IF=5.5] Purification and partial characterization of M1-UVs300, a novel bacteriocin produced by   isolated from fermented sausage Lactobacillus plantarum.

    Author: Yu An, Ying Wang, Xiaoyue Liang, Huaxi Yi, Zhaohang Zuo, Xiaoxi Xu, Dongjie

    Zhang, Changqing Yu, Xue Han

    Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

    Journal: Food Control  Volume 81, November 2017, Pages 211-217

    Institution:Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

    Paper linkhttps://doi.org/10.1016/j.foodcont.2017.05.030

     

    3. [2021 IF=5.75] The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System.

    Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Jingjing Yuan and Dewen Qiu

    Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

    Journal: Frontiers in Plant Science.  Volume 81, November 2017, Pages 211-217

    InstitutionInstitute of Plant Protection, Chinese Academy of Agricultural Sciences

    Paper linkhttps://doi.org/10.1093/jxb/ery351

     

    4. [2021 IF=2.99] Purifcation, characterization, and action mechanism of plantaricin DL3, a novel bacteriocin against Pseudomonas aeruginosa produced by Lactobacillus plantarum DL3 from Chinese Suan Tsai.

    Author: Xinran Lv,  Yang Lin ,Yu Jie, Mengtong Sun, Bolin Zhang,Fengling Bai,Hongfei Zhao,Jianrong Li

    Marker: RTD6127,超低分子量蛋白Marker IV(3.3-45 kD)

    Journal: European Food Research and Technology. Vol 244, pages 323–331 (2018)

    InstitutionCollege of Biological Science and Biotechnology, Beijing Forestry University

    Paper linkhttps://link.springer.com/article/10.1007/s00217-017-2958-3

     

    5. [2021 IF=6.99] Verticillium dahliae Alt a 1-like protein PevD1 targets cotton PR5-like protein and contributes to fungal infection.

    Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Dewen Qiu

    Marker: RTD6110,彩虹超低分子量蛋白Marker (1.2-45 kD)

    Journal: Journal of Experimental Botany, Vol 70, Issue 2, 15 January 2019,613-626

    InstitutionInstitute of Plant Protection, Chinese Academy of Agricultural Sciences

    Paper linkhttps://doi.org/10.1093/jxb/ery351

     

    6. [2021 IF=11.277] An  Alternative  Splicing  Variant  of  PtRD26  Delays  Leaf  Senescence by Regulating Multiple NAC Transcription Factors in Populus.

    Author: Hou-Ling Wang,Yi Zhang,Ting Wang, Qi Yang,Yanli Yang,Ze Li, Bosheng Li, Xing Wen, Wenyang Li, Weilun Yin, Xinli Xia, Hongwei Guo, and Zhonghai Li

    Marker: RTD6110,彩虹预染超低分子量蛋白(1.2-45 kD)

    Journal: Plant Cell. Volume 33, Issue 5, May 2021, Pages 1594–1614

    InstitutionBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University

    Paper linkhttps://doi.org/10.1093/plcell/koab046

     

    7. [2021 IF=2.89] Rapid and sensitive detection ofStaphylococcus aureus and Klebsiella pneumonia based on bacitracin-modified Fe3O4@PDA magnetic beads combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry.

    Author: Feng Qin, Shujuan Wang, Mingxia Gao  and  Xiangmin Zhang

    Marker: RTD6110,彩虹预染超低分子量蛋白(1.2-45 kD)

    Journal: Analytical Methods. 2021,13, 2804-2811

    InstitutionDepartment of Chemistry, Fudan University

    Paper linkhttps://pubs.rsc.org/en/content/articlelanding/2021/ay/d1ay00614b

     

    8. [2021 IF=3.74] Cloning, expression, and function of ferritins in the tick Haemaphysalis flava.

    Author: Yu Zhao, Lei Liu, Jin-Bao Liu, Cong-Ying Wu, De-Yong Duan, Tian-Yin Cheng

    Marker: RTD6142 高分子量非变性电泳蛋白质Marker II(45-669 kD)

    Journal: Ticks and Tick-borne Diseases. Volume 13, Issue 2, March 2022, 101892

    InstitutionCollege of Veterinary Medicine, Hunan Agricultural University,

    Paper linkhttps://linkinghub.elsevier.com/retrieve/pii/S1877959X21002454

     

    9. [2021 IF=0.886] Purification and Characterization of an Intracellular β-Glucosidase from the Protoplast Fusant of Aspergillus oryzae and Aspergillus niger

    Author: F.M. Zhu, B. Du, H.S. Gao, C.J. Liu, and J. Lia

    Marker: RTD6109 高分子量蛋白质Marker(43-200 kD)

    Journal: Applied Biochemistry and Microbiology, 2010, Vol. 46, No. 6, pp. 626–632

    InstitutionCollege of Food Science and Technology, Hebei Normal University of Science and Technology

    Paper linkhttps://link.springer.com/article/10.1134/S0003683810060116

     

    10. [2021 IF=4.374] Effect of Fuzhuan brick-tea addition on the quality and antioxidant activity of skimmed set-type yoghurt.

    Author: Dong Liu

    Marker: RTD6103 低分子量蛋白质Marker II(14.4-116 kD)

    Journal: International Journal of Dairy Technology, Vol 71 March 2018 Pages 22-33

    InstitutionDepartment of Food Processing Technology, Anqing Vocational & Technical College

    Paper linkhttps://doi.org/10.1111/1471-0307.12395

     

    11. [2021 IF=3.7] Smart delivery of poly-peptide composite for effective cancer therapy

    Author: Xin Yuan, Yingzhou Qin, Qingmei Tian, Cuijuan Liu, Xiangzhou Meng, Bo Qie, Fan Gao, Ying Huang, Guanghui Xu, Guang Yang, Yimin Zhu

    Marker: Protein Marker

    Journal: Biomedical Materials, 2022 Jan 24;17(2)

    Institution:School of Nano-Tech and Nano-Bionics, University of Science and Technology of China

    Paper linkhttps://pubmed.ncbi.nlm.nih.gov/34996052/

     


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